FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2638964
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTACG1456.635562E-494.922554250-254
AGTAGCT78100.078.0194245
CGAGTAG22700.077.5038763
GAGTAGC50350.074.997814
GTAGCTG79550.074.670836
CCGAGTA24150.073.140062
TAGCTGG83100.072.587437
CTACTCG15700.071.3447952
TACTCGG15950.071.101443
GCTGGGA157900.068.405529
GCTACTC35350.067.735571
AAGTGCT76900.066.0041665
TCTAGCG2951.083831E-465.70299280-284
AAAGTGC78700.065.117684
GCACTTT84050.064.153891
TTGGGAG105200.064.097986
CCCAAAG92300.063.8881721
CCAAAGT84250.063.115262
CAAAGTG83650.062.949833
CCCGAGT28100.062.649321
Produced by FastQC (version 0.11.5)