FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3197137
Sequences flagged as poor quality0
Sequence length35-301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC1100.0015010585243.54387290-294
ACGTATA3500.0018647482226.32867295
TATAGCG2753.45608E-11180.95154245-249
AAGCGTT4850.0049492097163.32997295
CGCCCGA2450.003369408161.66333295
CACCGTT3150.005397972158.73184250-254
GATCGTT3400.005972969153.44363275-279
TCGCATA1900.007990676139.26793285-289
CGCACGT1250.009090934133.4059250-254
TGCGTTA2000.009928982129.50821270-274
TACCGGG2250.009479337118.81125290-294
TATCGAC2257.2609546E-6100.5723130-134
TCGTATG2600.00607853498.35808265-269
GCGGTTG2700.002145810395.81288270-274
CTCCGTA2953.7455466E-488.42514275-279
CACGCCG3001.9478242E-586.23159270-274
GATCGGG3001.943796E-581.76434235-239
CGGGCGG6956.2651164E-476.14649285-289
GCCGGTT3454.597883E-675.926796280-284
CGGGCGT3304.7403766E-575.39647245-249
Produced by FastQC (version 0.11.5)