FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3490980
Sequences flagged as poor quality0
Sequence length35-301
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCCCG4151.1569164E-5261.9138280-284
TGACGTA3050.001852485226.88329240-244
CGTCGAA1150.002242138213.00507285-289
ATACCGG1801.0660008E-4204.12985285-289
GCGAAAT3500.0028224466197.10732235-239
TAATGCG1900.003968555175.9795170-174
GTTTCGG4750.006132173152.06435275-279
TTTGCGT4800.0071275528144.60945245-249
GACGTAA2400.007193384144.23923240-244
CGTATAC2400.008170911138.2233695-99
ATCGGGT4458.443682E-4124.735344295
GGCGGTA2852.2577633E-4118.967094200-204
CGCGGGT2951.0092481E-6115.98128225-229
AGCCCGT4953.6798647E-4110.02801280-284
CGTGCGT2100.0062350947106.18138170-174
GGTACGC1050.0019661105.52014145-149
CGTGTGA8252.3889199E-589.07485285-289
TTCGGGG6251.7234379E-788.81157295
AGCGGAC4002.358889E-586.499245240-244
AGGCGCG4003.1620273E-485.27726210-214
Produced by FastQC (version 0.11.5)