FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3490980
Sequences flagged as poor quality0
Sequence length46-301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACGT1654.7769463E-6327.14697285-289
TAAGCGG2507.571793E-5235.95836295
CACGCGT1100.0026783687200.72736150-154
CGCTACG1700.0016030972194.44124110-114
TATACCG1850.0031228878190.64981270-274
GGGGTCG6806.0505154E-5173.4988295
CGTTCGA1300.004269436171.7603180-184
GTGCGGA4350.0047644507165.45364285-289
TCCGTCG1550.005828456154.77924285-289
AGGCGTT4851.7032694E-4145.44418270-274
CGTGCAA4203.996669E-5140.4514295
TACGCCC2500.001703387139.90884260-264
GCGCCGA2600.009321297132.26157240-244
TGCGATC2800.009929404129.49045290-294
TTGCGGG4853.255991E-10121.62801295
GTCGTTT5052.5584296E-4116.811066295
TACTACG2957.243255E-5116.51005240-244
TGGTCGC6100.0108.37708105-109
TATGCGG3150.007471069105.142914150-154
TTGGGCG5707.606046E-6103.4905295
Produced by FastQC (version 0.11.5)