FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4178127
Sequences flagged as poor quality0
Sequence length35-301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG21800.066.023352
TACTCGG22650.064.1267553
CGAGTAG22350.062.9290853
GCTGGGA179650.062.3513769
GCTACTC52900.061.391931
AGTAGCT87700.061.086615
CCGAGTA23700.060.7303432
GAGTAGC55000.060.590884
GTAGCTG90400.059.7825746
TTGGGAG133350.059.016356
TAGCTGG96400.058.870637
GGAGGCT140950.056.978269
CCCGAGT27800.056.2823451
AAAGTGC106150.055.481734
CCCAAAG126900.054.6551171
CCAAAGT116450.054.011032
AAGTGCT107350.053.823865
TCGGGAG31000.053.2332846
AGTGCTG109700.052.5610736
GTGCTGG112150.052.2448357
Produced by FastQC (version 0.11.5)