FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2988410
Sequences flagged as poor quality0
Sequence length35-301
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG15450.063.616683
GCCCGAT2650.003378061662.990143270-274
CTACTCG14000.061.2016452
TACTCGG14750.060.2515073
AGTAGCT64500.059.219945
GCTAGCG2850.00437229359.017876275-279
GAGTAGC40700.058.710344
CTACGCT2850.00483076857.55023260-264
CCGAGTA17750.057.2602462
GCTACTC32450.056.2791021
GTAGCTG66400.056.1072046
GCTGGGA127450.055.599349
TAGCTGG71400.053.7442937
TTGGGAG94250.053.266076
AAAGTGC65850.052.106964
AAGTGCT67350.051.2959985
CCCGAGT21650.050.230011
CCCAAAG85600.049.6430551
GCGTATC1550.00884892449.38948220-224
CCAAAGT77150.049.172492
Produced by FastQC (version 0.11.5)