FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2988410
Sequences flagged as poor quality0
Sequence length35-301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG15600.075.02072
CGAGTAG17900.070.96653
TACTCGG16800.068.61193
CCGAGTA19000.066.857912
GCTACTC39950.065.963111
GAGTAGC40200.065.40114
AATCGCG800.005674885665.24925240-244
AGTAGCT63950.065.212535
GCTGGGA143850.061.2224779
GTAGCTG67900.061.0774736
TTGGGAG108250.060.9171336
TAGCTGG72550.059.853547
CCCGAGT23700.059.0702171
AAGTGCT76050.058.7040335
CCCAAAG89050.058.3926621
AAAGTGC75550.058.3889054
CCAAAGT81800.057.803672
GCACTTT84950.056.22541
ACTTTGG86300.055.7436183
GGAGGCT113550.055.006939
Produced by FastQC (version 0.11.5)