FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3028677
Sequences flagged as poor quality0
Sequence length35-301
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGCG1950.001361144879.217415290-294
CGAGTAG19250.072.245093
CCGAGTA20550.068.817042
CGTTTAG2250.002550084867.62333285-289
AGTAGCT69700.066.742055
GAGTAGC46250.065.78544
GTAGCTG74000.062.699666
CTACTCG14900.061.7746732
GCTGGGA143750.058.976799
TAGCTGG80100.058.4555057
TACTCGG15800.058.4238363
CCCGAGT25300.058.079691
AAGTGCT72550.055.6657145
TTGGGAG100050.055.054626
AAAGTGC71800.054.301794
GCTACTC35800.053.6482161
CCCAAAG89550.053.107611
CCAAAGT81950.051.9189952
GCGACGC2950.0074529551.577118285-289
AGTGCTG79150.051.3308876
Produced by FastQC (version 0.11.5)