FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3028677
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG18950.076.905543
CCGAGTA20800.072.6928252
AGTAGCT72000.067.9043965
GAGTAGC45900.067.4781654
GTAGCTG74800.065.125396
CTACTCG20000.064.670352
GCTACTC42650.064.363491
TACTCGG20300.064.312893
TTGGGAG112850.063.566156
CCCGAGT24900.063.171411
GCTGGGA156750.062.997659
TAGCTGG80250.062.9836167
AAAGTGC76700.062.789014
AAGTGCT78450.061.8569265
CCAAAGT86500.059.6001242
GCACTTT87400.059.3425981
CAAAGTG84350.059.319533
ACTTTGG88750.059.1838653
CCCAAAG97100.058.167961
TTTGGGA97950.057.9131165
Produced by FastQC (version 0.11.5)