FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3541092
Sequences flagged as poor quality0
Sequence length35-301
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGCCG2903.4627137E-6354.35248220-224
CGGCTAT1854.9298245E-4353.0329395-99
CGTCGAG1251.7521779E-7267.61618175-179
CGTCGCC2800.0016860822234.1459115-119
CGCGTTG1600.0010484165205.07373130-134
CGTATCG1100.0027518084198.9228130-134
ACGCCGC3800.0028166145197.22937285-289
CGGAGCG3550.0032094333188.83055180-184
ACCGCCG2052.882961E-4160.51836140-144
GTACGGC2303.6297693E-5154.64696255-259
ATGCGGT4900.0076742633141.081225-229
GCGATAG2550.0021836422140.94762265-269
TCGATAC1850.009328903132.24565275-279
TGCCGCG1750.009661115130.70932220-224
ACCGGGG4701.2190465E-4125.35032295
TGTCGGG5300.0041403268111.159706295
AATCCGG3406.964116E-5109.26112280-284
TATAGCG3206.542905E-9105.671616200-204
CCGTACC3201.2281453E-7102.04858495-99
TAGAGCG3550.001432382398.961914245-249
Produced by FastQC (version 0.11.5)