FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3541092
Sequences flagged as poor quality0
Sequence length35-301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGTT3807.5208204E-4306.37396295
ATTGGCG3700.002794504197.75388285-289
GAGCGTG6650.004015998175.07085295
GCGAAGC3900.004487671168.81523145-149
GCCGGTC4200.0045306277168.26656260-264
TATCGGG2000.004736489167.82854190-194
ATACGGC2100.0051075374161.7433210-214
CCGATGC4150.0052497615160.18889170-174
TAAGCGG2200.008894449150.50163165-169
CGCCCGT2550.0015309133144.8644290-294
TGTCGGG5400.00840989136.81638290-294
ACGAGTG5250.009217245132.69215245-249
GCTACGC1353.3977418E-4127.31905225-229
CGGGCTA2905.8176345E-5121.8482260-264
TAGACGG3202.4233486E-6101.793045100-104
GGACCGA3754.7435606E-894.03478255-259
ATTGCGT1903.821291E-493.912025270-274
CGAACGA1302.6315422E-493.90686285-289
GGGTTCG3652.0008883E-1193.52261220-224
CCCTATA7803.108653E-993.306816280-284
Produced by FastQC (version 0.11.5)