FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3196480
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG24050.070.13793
AGTAGCT83700.067.466015
CCGAGTA25850.066.9169162
GAGTAGC56150.066.650674
GTAGCTG87800.065.073016
TAGCTGG94500.062.0588237
GCTGGGA173450.061.104229
GCTACTC39200.059.7770841
CTACTCG15550.059.7190172
CCCGAGT30550.058.4210851
AAAGTGC80050.058.0802654
CCAAAGT88100.057.250172
AAGTGCT83000.057.1086045
TACTCGG16550.056.988893
TTGGGAG108850.056.37696
GCACTTT88350.055.930841
CCCAAAG98500.055.7758031
CAAAGTG88350.054.2663043
CGCCTAA2850.0063099653.79524280-284
GACGTAC2950.006571240353.245968290-294
Produced by FastQC (version 0.11.5)