FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3069862
Sequences flagged as poor quality0
Sequence length35-301
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG21950.074.785653
CCGAGTA23450.069.237392
AGTAGCT83250.068.8292855
GTAGCTG85900.066.2764746
GAGTAGC55350.065.757554
CGCCTAA2200.002877117865.59695280-284
TAGCTGG92200.063.348197
GCTGGGA167550.063.0038959
TACTCGG14550.060.424713
AAGTGCT83000.059.9255455
CTACTCG14350.059.7985532
CCCGAGT26900.059.675841
CCAAAGT90750.059.3875432
GCTACTC36150.059.265051
CCCAAAG100650.058.88911
AAAGTGC84900.058.2947924
TTGGGAG109300.057.487356
CAAAGTG90150.056.8094833
CGGGTAA3950.00571504255.153946295
GCACTTT87900.054.998441
Produced by FastQC (version 0.11.5)