FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3069862
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG19550.076.712482
CGAGTAG19300.074.5214843
AGTAGCT77150.073.5436865
TACTCGG20700.073.341463
GAGTAGC50550.072.964184
GTAGCTG79200.071.030746
CCGAGTA21500.070.612482
GCTACTC46250.070.583781
TAGCTGG86050.067.47597
TTGGGAG125000.066.6468666
GCACTTT101300.065.9693451
AAAGTGC82900.065.773164
GCTGGGA168350.065.635879
ACTTTGG101550.063.673513
AAGTGCT86600.063.6728325
CCAAAGT92800.063.5175252
CCCGAGT25150.062.33391
TTTGGGA115050.060.8578645
CCCAAAG104350.060.8008041
CAAAGTG92650.060.701393
Produced by FastQC (version 0.11.5)