FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2816411
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAA1654.455178E-6124.99964295
AGTAGCT93200.082.689715
CGAGTAG25800.081.6910253
GTAGCTG95900.080.3602146
GAGTAGC60500.079.057314
CTACTCG19300.077.826882
TAGCTGG103700.076.300257
GCTGGGA188350.075.728669
ACGCGTT1300.001642246775.56409265-269
CCGAGTA27150.075.513932
AAGTGCT94900.073.431575
TACTCGG20750.072.844893
AAAGTGC97700.071.1091844
TTGGGAG125050.070.877536
CCAAAGT103600.070.775332
GCTACTC48550.069.237741
CAAAGTG103400.068.287333
CCCAAAG118650.066.566231
CCCGAGT31500.066.2440951
AGTGCTG103000.065.726586
Produced by FastQC (version 0.11.5)