FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2435081
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG17000.087.226923
CTACTCG19500.085.025762
CCGAGTA18150.083.180482
TACTCGG20150.081.284553
AGTAGCT66200.080.249675
GCTACTC42900.078.893141
GAGTAGC42350.077.9961244
GTAGCTG70100.075.603526
GCTGGGA147900.073.118329
TAGCTGG76250.071.999467
TTGGGAG105650.070.2038046
AAAGTGC78700.070.151924
AAGTGCT80650.068.713565
GGAGGCT117650.067.892319
CCCAAAG93450.067.528861
CCCGAGT23250.066.695691
CCAAAGT86400.066.462382
ACTTTGG82550.065.728793
GCACTTT81550.065.560471
TCGGGAG26600.065.399676
Produced by FastQC (version 0.11.5)