FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2985871
Sequences flagged as poor quality0
Sequence length38-301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCC1454.3248798E-4368.8151285-289
TACGCCG856.964846E-4314.7221285-289
CAAGCCG3400.0016123084237.56935295
ACGACCG1200.0026322538201.87796135-139
CTCGAAA4300.0032560744187.84552295
GTCGATT1450.008124097185.25813290-294
CGTATCT3050.0039522317176.14708290-294
GTACTCG2550.0014193157158.37958295
TACGGAG3550.0063096494150.64279285-289
CCCTGCG5650.0073680175142.96208295
ACCGTAT3000.0048014643134.62263295
TCGTAGC1850.0026251841134.07097195-199
GCTACGG2100.006095329122.81781255-259
CGTTATC2357.6593424E-4112.13584275-279
CGGTTAG2401.9597539E-5109.79968275-279
GGTACCG2450.001044244109.13917285-289
CGTAAGG2404.8797025E-5102.56014185-189
CCGGTAC2651.684728E-6100.166405280-284
TAGGCGC2504.980775E-697.06292145-149
CAATGCG2656.141523E-695.467026230-234
Produced by FastQC (version 0.11.5)