FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3179238
Sequences flagged as poor quality0
Sequence length35-301
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCGA1203.5880684E-4110.79806150-154
CGTTACG750.005866495476.06124280-284
CCGAGTA14550.057.3752442
CGAGTAG14600.055.7317353
AGTAGCT54850.051.6507455
GTAGCTG55400.051.3523836
TAGCTGG59500.050.5089577
GAGTAGC34500.050.268814
AGACGTA4704.2212885E-449.898968290-294
GCTGGGA105400.049.4189879
CCCGAGT19350.047.4636341
CTACTCG10400.046.4420282
CGATCGC1252.742266E-445.078373275-279
TACTCGG10700.044.9610143
TTGGGAG79800.044.209796
AAGTGCT61400.042.755185
CCCAAAG76650.042.5594861
GCTACTC28250.042.2214131
AAAGTGC61200.041.924434
GGAGGCT79050.041.7741629
Produced by FastQC (version 0.11.5)