FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4114958
Sequences flagged as poor quality0
Sequence length35-301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGG1851.3368754E-5100.21613295
CGAGTAG36400.094.742733
AGTAGCT131700.092.632885
CTACTCG38150.090.394532
TACTCGG38650.090.391143
CCGAGTA37950.089.883212
GAGTAGC83950.086.899754
GTAGCTG139550.086.5212556
GCTACTC85200.082.6609341
TTGGGAG212600.082.578116
TAGCTGG151150.082.427637
AAGTGCT147750.080.641725
GCTGGGA300600.080.0955359
GCACTTT158900.079.439141
AAAGTGC152250.077.168164
ACTTTGG164150.076.865723
TTTGGGA183850.076.757255
CCCGAGT45750.075.084221
CACTTTG167900.075.013172
CCAAAGT163450.074.716252
Produced by FastQC (version 0.11.5)