FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3209805
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA11100.040.6630172
CTACTCG10600.039.032662
CGAGTAG10600.039.032663
TCGCGTT853.9495717E-738.7171555
CCCGAGT15600.037.9992451
GCTACTC23650.037.9954221
TTGGGAG67700.037.6164476
TACTCGG11350.037.28193
GCTGGGA78950.037.021399
GTAGCTG41150.036.652396
AGTAGCT42300.036.3439145
GAGTAGC26400.034.72714
GGAGGCT61650.034.147549
GGGAGGC85500.033.249898
TAGCTGG48700.032.7083177
CGGAGGT13300.032.149727
GCACTTT60700.030.8477441
TCGGGAG13350.030.6199976
ACTTTGG61300.030.3728873
CACTTTG63150.030.1531752
Produced by FastQC (version 0.11.5)