FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9816828
Sequences flagged as poor quality0
Sequence length100-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG66650.073.9554753
CTACTCG66600.073.049942
GCTACTC162300.068.861951
CGAGTAG46100.068.602713
CCGAGTA49350.065.454492
GCTGGGA449500.065.146689
GAGTAGC118700.063.9332734
GGAGGCT371350.063.662499
AGTAGCT191650.063.211165
GTAGCTG195900.061.9613346
TCGGGAG83450.061.352716
CTCGGGA80750.060.632215
AAAGTGC303100.058.3700034
AAGTGCT312100.057.4711655
ACTCGGG84600.057.24734
TTGGGAG290250.056.9525456
AGTGCTG315500.055.6023676
TAGCTGG222750.055.5913547
GTGCTGG313600.055.2431687
CCCGAGT63200.055.170761
Produced by FastQC (version 0.11.5)