FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3413926
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG1201.8189894E-1243.181011
GCTGGGA71300.030.5174039
CGAGTAG11600.030.4237753
TCGCGTT1401.8739229E-830.2463785
TTGGGAG58200.030.0440676
CCCGAGT16250.029.858311
CCGAGTA11500.029.463392
GAGTAGC27100.029.3410934
AGTAGCT39150.029.2033985
TACTCGG8600.028.4521263
GTAGCTG39800.027.8719736
CTACTCG8450.027.8459052
CCCGAAT7850.027.0037611
GCTACTC22300.026.8275031
GGAGGCT56650.026.3805189
CGGAGGT9650.026.3018427
GGGAGGC71000.026.2816038
TAGCTGG44350.025.647297
TCGGGAG12650.025.2672026
TGGGAGG75500.024.5906437
Produced by FastQC (version 0.11.5)