FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3120136
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG12600.043.687243
CCGAGTA12800.043.006012
AGTAGCT50700.042.589475
CTACTCG10700.042.212462
GCTGGGA91950.041.4509939
TTGGGAG73000.040.9468886
GAGTAGC33850.040.8588524
TACTCGG11750.040.841493
GTAGCTG52250.040.4774256
CCCGAGT16700.038.078471
GGAGGCT67050.038.0598349
TAGCTGG57250.037.680527
AAAGTGC46900.036.4108924
TCGCGTT1055.075708E-835.842495
GCACTTT65350.035.8239061
GGGAGGC96900.035.745678
CTCGGGA14050.035.156895
CCCAAAG63600.034.587721
AAGTGCT50050.034.4952665
TCGGGAG15150.034.4347576
Produced by FastQC (version 0.11.5)