FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8851076
Sequences flagged as poor quality0
Sequence length100-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG40850.069.7774663
TACTCGG56100.068.797153
CTACTCG57200.066.813212
CCGAGTA43750.065.927172
GCTACTC138900.064.227521
GCTGGGA393500.062.2951669
GAGTAGC104000.061.6811684
AGTAGCT170950.059.9858065
GGAGGCT329450.059.2545979
TCGGGAG71550.057.8645486
GTAGCTG177850.057.7928626
CTCGGGA68250.056.16385
TTGGGAG257800.056.1329546
CCCGAGT58350.055.4638561
AAGTGCT270250.054.528935
TAGCTGG197350.053.9738777
AAAGTGC267350.053.9194874
GTGCTGG268150.053.208417
CCCAAAG324450.052.6862341
ACTCGGG72400.052.5824474
Produced by FastQC (version 0.11.5)