FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9895589
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG39600.071.287733
CCGAGTA42200.068.6273962
TACTCGG52600.066.092493
CTACTCG53400.062.5584372
GCTACTC134850.060.772451
GCTGGGA381750.059.043189
GAGTAGC104850.058.4590644
CCCGAGT56900.057.749041
GGAGGCT315800.057.2849469
AGTAGCT174650.055.898415
TCGGGAG69150.054.1372836
GTAGCTG179400.053.424666
CTCGGGA66700.052.511745
TTGGGAG258450.051.8975036
AAGTGCT271000.050.010015
GTGCTGG268400.049.4867487
AAAGTGC267050.049.4528354
TAGCTGG201700.049.42917
AGTGCTG271850.049.1951836
ACTCGGG69700.048.5644
Produced by FastQC (version 0.11.5)