FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9937526
Sequences flagged as poor quality0
Sequence length100-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG55000.080.994093
CTACTCG84200.079.19382
TACTCGG87300.078.113823
GCTACTC200000.075.4562761
CCGAGTA60500.075.434842
GCTGGGA568400.071.134569
GAGTAGC142200.070.8964844
AGTAGCT229850.070.2069555
AAGTGCT371850.066.833465
GTAGCTG240450.066.8222666
GGAGGCT474650.066.784639
TCGGGAG108900.066.729466
CTCGGGA105650.066.257445
AAAGTGC367050.066.0832064
CCCGAGT75700.063.37911
TTGGGAG353700.063.3587466
CCAAAGT406500.063.3398442
ACTCGGG107400.062.7453544
AGTGCTG385750.062.712996
CCCAAAG449150.062.254111
Produced by FastQC (version 0.11.5)