FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10296069
Sequences flagged as poor quality0
Sequence length100-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG56550.080.6730963
TACTCGG92250.080.243553
CTACTCG90950.078.7691042
CCGAGTA60700.077.063312
GCTACTC220550.075.9416661
GAGTAGC150350.075.6999744
AGTAGCT248850.075.024855
GCTGGGA622550.072.318939
GTAGCTG259750.071.271616
GGAGGCT518600.067.971779
TCGGGAG117650.066.892896
AAGTGCT404550.065.888185
CTCGGGA114950.065.211915
AAAGTGC402700.065.142864
CCCGAGT77650.064.726621
ACTCGGG114400.064.295724
TAGCTGG297350.064.015947
TTGGGAG383300.063.9552656
CCAAAGT437650.063.7624052
AGTGCTG417700.063.1940236
Produced by FastQC (version 0.11.5)