FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10296069
Sequences flagged as poor quality0
Sequence length100-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG59700.084.3843843
CTACTCG98100.081.605132
TACTCGG100850.081.1073
CCGAGTA64100.079.637122
GCTACTC224150.077.899081
GAGTAGC155450.077.139854
AGTAGCT244850.076.8438345
GCTGGGA622950.074.646829
GTAGCTG259450.072.871146
TCGGGAG125250.069.6196066
GGAGGCT524400.069.504549
AAAGTGC400250.068.53924
CCCGAGT80750.068.2895051
CCAAAGT431550.067.449272
CTCGGGA123150.067.4442145
AAGTGCT411950.067.363995
TTGGGAG390650.066.4841846
ACTCGGG122150.066.3054664
CCCAAAG476700.066.117861
TAGCTGG298700.065.584577
Produced by FastQC (version 0.11.5)