FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2979969
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG10600.040.830413
CCGAGTA11000.038.9199642
TTGGGAG61950.037.7071046
CCCGAGT14650.035.0359541
TACTCGG8350.034.930683
CTACTCG8750.034.4108662
AGTAGCT42650.034.3027235
GCTGGGA77650.034.0746429
GAGTAGC26950.033.863374
GTAGCTG43550.033.3486636
TAGCTGG46650.032.9461147
GCTACTC19250.032.779261
GGGAGGC76000.031.9731228
GGAGGCT58400.031.4650389
TTTGGGA66000.030.5774025
GCACTTT57600.030.2485331
CCCAAAG53600.029.9581261
TGGGAGG80600.029.5652147
CTTTGGG64150.029.4792634
AAGTGCT42650.028.7878155
Produced by FastQC (version 0.11.5)