FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2727073
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA10850.040.742932
CGAGTAG11000.039.33233
TTGGGAG59400.037.1000146
TACTCGG8950.036.781483
CCCGAGT13650.036.5452041
GCTGGGA68300.036.535029
GAGTAGC24950.036.3781784
AGTAGCT37750.036.0013775
GTAGCTG38050.035.935696
GGAGGCT56250.034.2528579
TAGCTGG42400.034.0239037
CTACTCG8650.032.6204032
TTTGGGA58400.032.0485575
GCACTTT52000.032.0373841
GGGAGGC78450.031.3864358
TCGCGTT600.004150714331.3506835
CCCAAAG47950.030.6212641
CGCCGTA1551.5206751E-930.32267494
TGGGAGG76150.029.8049817
ACTTTGG54300.029.7929023
Produced by FastQC (version 0.11.5)