FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8934692
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG50400.070.516833
CTACTCG50400.068.978282
CGAGTAG35850.065.970923
CCGAGTA38500.064.283372
GCTACTC123950.061.532631
GCTGGGA333650.058.9005439
CTCGGGA60600.057.8918345
GAGTAGC93650.057.753174
GGAGGCT291150.057.180629
TCGGGAG64750.057.167166
AGTAGCT153100.056.379525
CCCGAGT52450.056.1939241
GTAGCTG156000.054.7028586
TTGGGAG241950.053.0522776
ACTCGGG66550.052.821294
AAAGTGC232500.050.7777864
TAGCTGG174700.050.7713977
AGTGCTG230450.049.7008486
AAGTGCT240000.049.183275
GTGCTGG229800.048.9710737
Produced by FastQC (version 0.11.5)