FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9619174
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG52300.065.478292
TACTCGG52350.064.7951053
GCTACTC135400.061.4913021
CGAGTAG34850.060.785953
CCGAGTA37150.060.171112
GCTGGGA358800.058.444729
GAGTAGC95800.058.1982774
AGTAGCT155350.057.0475925
GGAGGCT312400.056.6656279
CCCGAGT51100.054.438491
GTAGCTG163750.053.7936826
TTGGGAG261850.053.015746
TCGGGAG64600.052.8939676
CTCGGGA64750.051.878215
TAGCTGG186750.049.9873777
AAGTGCT254800.049.8263785
AAAGTGC254900.049.328684
CCCAAAG304750.049.0958561
GTGCTGG250050.048.556427
AGTGCTG254100.048.394826
Produced by FastQC (version 0.11.5)