FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3364816
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGGAG68350.036.650076
CGAGTAG12600.035.098013
GAGTAGC30300.034.3091744
AGTAGCT46900.034.0006685
CTACTCG8850.033.4940532
GTAGCTG46450.033.288666
CCGAGTA13600.032.8666272
TACTCGG9450.032.3598673
GCTGGGA88950.031.9651079
GCTACTC23800.031.6663721
TCGCGTT753.260863E-431.3595165
TCGCGAA600.004163570231.33108794
GGAGGCT66250.031.0000449
TAGCTGG52700.030.6780327
GGGAGGC87400.030.650898
GCACTTT61200.029.9401151
TTTGGGA73550.029.419535
CCCGAGT16750.028.9652771
CGGAGGT13950.028.2998897
CACTTTG66150.027.8110472
Produced by FastQC (version 0.11.5)