FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3364816
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG10950.048.9448052
TACTCGG12750.043.1411363
GCTACTC29050.041.4578551
TTGGGAG78150.041.003376
GCACTTT69350.036.8354841
CACTTTG70000.036.535642
TTTGGGA76950.035.9218525
GGAGGCT77150.035.6946569
AGTAGCT48800.035.4501465
ACTTTGG70500.035.142883
CCGAGTA12100.034.9681782
TCGGGAG15600.034.9379546
GCTGGGA87750.034.7567449
CGAGTAG12200.034.29623
GAGTAGC31300.034.095024
CTTTGGG79000.033.9201054
GGGAGGC105500.033.8509148
GTAGCTG49850.033.2715956
CCCGAGT15500.032.7797051
CTCGGGA15700.032.0386475
Produced by FastQC (version 0.11.5)