FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11227312
Sequences flagged as poor quality0
Sequence length100-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG83800.077.2508853
CTACTCG82600.076.792712
CGAGTAG49100.072.840683
GCTACTC193750.070.929831
CCGAGTA53550.069.552932
GCTGGGA537600.065.892349
GAGTAGC132600.065.5451354
TCGGGAG104750.065.538566
AGTAGCT219300.065.0206765
GGAGGCT462900.064.485819
CTCGGGA103400.063.830265
GTAGCTG224700.063.7129446
ACTCGGG105200.061.155414
AAAGTGC366600.060.3504754
AAGTGCT372500.060.2170265
CCCAAAG446350.058.6036571
CCCGAGT71400.058.209421
TTGGGAG356850.057.6587266
CCAAAGT414250.057.5333332
GTGCTGG376500.057.1605877
Produced by FastQC (version 0.11.5)