FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11227312
Sequences flagged as poor quality0
Sequence length100-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG82050.075.3209843
CTACTCG81250.073.931552
CCGAGTA53400.072.207762
CGAGTAG52750.072.087553
GCTACTC200000.071.672691
GCTGGGA536700.068.979529
GAGTAGC131950.067.680584
AGTAGCT217650.065.3813255
GGAGGCT460000.064.196729
TCGGGAG104250.063.6772736
GTAGCTG227950.062.742246
CTCGGGA99250.062.1952365
AAGTGCT374750.062.120895
AAAGTGC370550.061.284724
ACTCGGG102300.060.476244
AGTGCTG380550.059.960856
CCCAAAG443350.059.947021
CCCGAGT69450.059.2730981
CCAAAGT408500.059.1455152
GTGCTGG389500.058.618297
Produced by FastQC (version 0.11.5)