FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10074067
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG54000.066.3482363
CTACTCG54650.064.033682
CGAGTAG41850.061.7953033
CCGAGTA44600.061.6597822
GCTACTC135200.061.2953721
GCTGGGA369400.057.540789
TCGGGAG69550.056.8378646
GGAGGCT312700.056.8326769
GAGTAGC102100.056.6548274
AGTAGCT167200.055.0497745
CTCGGGA67450.055.048135
TTGGGAG261250.052.1995286
GTAGCTG174350.052.0325786
CCCGAGT60350.051.7915461
ACTCGGG72400.050.2048074
TAGCTGG192650.049.3200877
AAAGTGC266450.048.2593084
AAGTGCT270500.048.1628765
CCCAAAG324050.047.7844281
AGTGCTG267900.047.4518626
Produced by FastQC (version 0.11.5)