FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2814119
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT44700.043.25855
CCGAGTA12150.042.6005632
GTAGCTG45400.042.44416
CGAGTAG11800.041.4700743
GAGTAGC29250.041.176674
TAGCTGG49300.039.9430277
TTGGGAG65000.039.9130636
GCTGGGA83600.039.35059
CCCGAGT15550.038.782441
TACTCGG8750.037.642073
CTACTCG8300.035.715882
GCACTTT56750.035.2840651
ACTTTGG56700.034.8537643
TTTGGGA66150.034.4944345
GCTACTC21650.034.383871
CCCAAAG54300.033.8392221
AAAGTGC41750.033.6939434
GGGAGGC82350.033.2149248
TCGCGTT851.7435714E-533.2100455
GGAGGCT58300.033.1307839
Produced by FastQC (version 0.11.5)