FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2814119
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG11300.050.7631383
CTACTCG11450.048.8661962
TTGGGAG72650.045.5289466
AGTAGCT42550.044.7514465
GAGTAGC27450.043.6774374
CCGAGTA11650.043.1842042
CGAGTAG11550.043.151013
GCACTTT62900.043.0986631
GCTGGGA87600.041.8440489
GTAGCTG46250.040.33016
AAAGTGC42850.040.159624
TTTGGGA71350.040.130535
CACTTTG66300.039.9265672
ACTTTGG65300.039.3859373
TAGCTGG50850.038.8110357
TCGGGAG15700.038.604746
GGAGGCT70800.038.4979749
CCCGAGT14600.038.360661
AAGTGCT47150.038.0919575
GGGAGGC100600.037.042078
Produced by FastQC (version 0.11.5)