FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11062093
Sequences flagged as poor quality0
Sequence length100-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG52200.078.598893
TACTCGG82000.075.617273
CCGAGTA56450.075.612052
CTACTCG83600.073.774272
GCTACTC196850.071.082551
GAGTAGC136200.069.575654
GCTGGGA533200.068.77379
AGTAGCT222750.067.247395
GTAGCTG233250.063.927276
GGAGGCT470200.063.7076269
TCGGGAG103600.062.5139356
CCCGAGT72700.062.457291
CTCGGGA101250.061.948395
AAAGTGC363050.060.806634
TTGGGAG370150.060.3362926
AAGTGCT371200.060.2634435
ACTCGGG103700.059.1551134
TAGCTGG259500.059.1436967
AGTGCTG379200.058.547396
CCAAAGT398500.058.5031932
Produced by FastQC (version 0.11.5)