FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3138750
Sequences flagged as poor quality0
Sequence length39-301
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTA600.00769459361030.4194185-189
CGTAACG650.008918535957.07635190-194
CGGTAGG2301.5176731E-4522.5269120-124
CATGACG2903.0399443E-4414.41785115-119
GCATCGG3253.6421904E-4390.1149235-239
AATCGTG3555.021334E-4350.4787195-199
ACTCGCG1900.0036187903335.78058255-259
GCGAAAT3655.8395206E-4333.27078160-164
CGGTTTA2008.3488127E-4299.85205110-114
TAGGCGG2051.51606455E-5296.0917155-159
TACGGTC2250.0011369694267.0693120-124
GGTTGCG2400.007281584249.37796105-109
CCTACCG1650.0015048974243.2778135-139
AAGCCGA5050.0015066552242.84926175-179
ACCGGTT2601.1611794E-4241.27135220-224
GCGGGTT2708.997758E-6231.36732215-219
ATCGACT2751.9680889E-4229.55179230-234
GAGCGAT2800.003368999215.04019135-139
CCGGAGG6050.0016613946209.56584235-239
GCCGTTG3155.665968E-4201.24974240-244
Produced by FastQC (version 0.11.5)