FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3138750
Sequences flagged as poor quality0
Sequence length39-301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA350.00272780661730.868165-169
CGTACCG650.009102098947.37933185-189
TCGCGTT1006.202754E-10630.0871250-254
CTACCGT2401.4576722E-4529.5413260-264
TCGTACC1500.0053997277420.0581240-244
TCTAGCG1855.349991E-4343.48627265-269
ACGGGTC1956.184237E-4327.2642270-274
TACCGTC2057.276041E-4309.9754260-264
CCTACCG2103.9283815E-4305.1924275-279
CGCAACC2100.009770095295.06155190-194
ACGGAGG7301.05501385E-10285.10458295
CGTTGCG1150.0010938768270.66754215-219
ATTCGTC2400.0012194249260.88513225-229
CGCTGAA5100.0013590157251.33495275-279
CCGATGG4255.1892734E-5244.85452295
GACGCGG2550.0015085465242.99187200-204
CGGCGGG4456.4010637E-6233.84982295
ATAGCGG2800.003443593230.87607280-284
CAACACG5600.001798149228.8943275-279
CGACAGG4651.0460486E-5223.79176295
Produced by FastQC (version 0.11.5)