FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3876691
Sequences flagged as poor quality0
Sequence length38-301
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTA500.0071621361068.3804185-189
TCGCGCG550.00881597962.9418150-154
AGCGCCG2703.5429833E-4393.99576170-174
AGTCGCA3004.1631903E-4373.31543280-284
GGTTGCG2850.0018270799300.84857295
GTCGCAC2250.0013990918249.25288280-284
CGATACC2352.2452423E-6224.0906595-99
ATACACG5500.002919465194.8116190-194
CTCGTAC2900.0029366869194.56148285-289
CCTGTCG4458.9615403E-4192.67831295
TACCGCC3004.142854E-4183.06816255-259
CGAGTAC3200.008941753174.99161280-284
TCGCAAA3350.0052801534159.91997190-194
CCCTCGT5258.6304615E-5158.32077270-274
TAGCGGT3702.4720066E-9147.99577250-254
TTCGACC2450.0073367124143.2960795-99
CGCGGGC3950.003810187142.8426285-289
TCCGCCG3951.0023177E-7141.76537280-284
CACGCAA3850.003081889141.39551225-229
GACGGGT4053.9285835E-4134.02215-219
Produced by FastQC (version 0.11.5)