FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3876691
Sequences flagged as poor quality0
Sequence length38-301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTT850.008318101990.92834295
GGATCCG3451.8579929E-4488.28354295
CGCGAGT1257.1243467E-6423.19302165-169
ATCGCGA1350.0048626475406.98355270-274
GGCGGTA3054.7562344E-4357.0969250-254
TGACGAC3657.4802036E-4306.9862290-294
TAGCGGA2200.009584564252.3648280-284
CCGGTTA2852.5320024E-4184.55486120-124
CCGTAGC3052.8701437E-5182.03363280-284
GTACGTA3050.004080442174.31259185-189
CGTAAGG3200.009159095169.6792240-244
TAGGCGG3259.43146E-9168.5538260-264
CGCGGGG5052.8808689E-4166.78992295
CTCTACG5100.0033282286165.15471295
AGCGGCG3350.004895879164.0083270-274
CTGAACG5300.0047084126158.92247295
TCGCCTT5008.698045E-5158.01918130-134
CCGTACG1150.0036220127157.3836235-239
AGCGCGA1400.006005866153.24501200-204
GGTTCGC2500.0064367894149.7024290-294
Produced by FastQC (version 0.11.5)