FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3432662
Sequences flagged as poor quality0
Sequence length47-301
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACG300.00226103171901.4656270-274
CGTACGT859.3571525E-61323.8245255-259
AACGCGA450.005482641221.0079160-164
CGCGTAA600.00990887908.16266100-104
GGTTGCG2101.3445398E-4544.2207280-284
CGCGTTG1551.322147E-6351.546895-99
TTGAGCG3305.714102E-4335.80994210-214
CCCGACG2750.0028023596324.67712295
CGAACAT4059.175319E-4286.6756290-294
GCGTACG950.0016345159236.73277250-254
ATTGCGC2608.539332E-5211.32831170-174
TATAGCG2708.8322195E-6204.52739195-199
GTTGCGG2900.002826371197.04543280-284
GTACGTC2900.0029609422194.00877255-259
CGCTCTT6000.0034220112184.69548205-209
CCGTTAA3101.8199735E-6178.43665215-219
GTATACC6400.0038043447178.2624275-279
CTCCGGT3200.008975253173.44449220-224
AGGACCG3200.0041612624173.15201210-214
CGGTGAG6856.45806E-4167.71642285-289
Produced by FastQC (version 0.11.5)