FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3432662
Sequences flagged as poor quality0
Sequence length47-301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAA650.00445595571353.735295
CGCGACA700.0051676581257.0398295
CACGCGA450.0055984411208.317495-99
CGCGATA250.006711681103.9904200-204
TTCGCGA550.0077350131027.895275-279
CGCGCTA600.009428792930.9816245-249
CGTACCG650.0057185953840.7169155-159
CTCGCGT1452.2549963E-5374.37314115-119
TCTACGC1750.0021066645310.7101795-99
GCCGTAC1907.949235E-6299.61844285-289
AAGCCGG3758.2627183E-4296.90082240-244
AGCCGCG2100.0011929981262.85486200-204
TAGGACG2154.622189E-4256.74194210-214
ACGCCGG2205.372749E-8250.9069205-209
TTCGGTA4500.0015382905241.2627110-114
ACGCTAG2450.0015134426230.75194280-284
TTTCGGC4800.0017748795229.99799215-219
CTAACCG2601.7806215E-6215.20917250-254
CCGCTAT3050.006430612187.62646290-294
ACGTCCG1550.0032893217187.38481290-294
Produced by FastQC (version 0.11.5)