FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3430965
Sequences flagged as poor quality0
Sequence length60-301
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCG450.00302531691643.3497180-184
AACGCGT450.00306656231632.271150-154
CGCGCTA500.0037010391485.7374185-189
ACGTACG600.0050893371266.908250-254
CGATCCG700.00725273971061.2411195-199
ACGCGTA350.0074269921049.317150-154
ACGATCG750.008249903995.0144205-209
CGTACGT400.009035604951.2872250-254
CAACGAG3608.60399E-5630.52136295
GTTGCGT2609.65778E-5607.3248290-294
GGACCGC2459.972523E-5600.9602165-169
CGGGTCC3352.1096207E-4467.96555285-289
GACCGCG1402.2642726E-6394.38013165-169
TAGAGCG2400.0010792629306.0508140-144
AGCCGGT3153.96185E-5241.31581250-254
ACGGTTG3450.0012435906228.84706290-294
CGGGGAT3308.910507E-4223.08371165-169
ACCGCGC3551.4351826E-9222.40067290-294
CCGACCA3350.0019831148221.75186185-189
TATACCG1850.0021450508216.12515290-294
Produced by FastQC (version 0.11.5)