FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3430965
Sequences flagged as poor quality0
Sequence length44-301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACG550.0042524051386.0043275-279
ATCGCGT600.00520383851252.8988250-254
ACGCGTC600.00542260851227.3872180-184
GCGTCGA650.0062204651145.9359205-209
AGTCGCG750.0078320381021.1862280-284
TTCGGCG800.009121806946.2299265-269
CGACGTT800.009725946916.3939170-174
ACTAACG2551.14615366E-4573.7001140-144
TCCCCGG5052.0231129E-4473.94165295
ATTGCGC3552.4992187E-7419.6385205-209
TCGCGTT901.1988959E-8418.11575250-254
TATAGCG1850.0031483916402.62616200-204
CCGTCAT4154.29626E-4369.10342280-284
CCCGAAC2104.8581767E-4354.69443210-214
TAGGCGT2508.302959E-4296.586195-199
AGGGCCG5258.8742183E-4289.72238270-274
CGAAATC5250.0010042277278.02954105-109
CGGGCTT4404.087069E-7271.97787295
TCGACGG1150.0011249124268.16312285-289
AAGCGAA5800.0011959206262.24872270-274
Produced by FastQC (version 0.11.5)