FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2874714
Sequences flagged as poor quality0
Sequence length46-301
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTA2450.002485013205.70016190-194
CCGTTCG1350.0033369039186.48674185-189
CGATGAT6150.007237721143.79132295
CACGCGA700.007568493141.85468155-159
ACCGAGT3800.00855912136.03555230-234
GATCGGA3300.009595943133.98737295
GACCGTA2050.0019693119122.15221180-184
GCGATAG2400.00551772117.642525290-294
AACGATC2300.003341601114.80413250-254
CCGATAA2204.4184236E-4112.42213145-149
TAGGCGG2551.9683284E-7103.54881250-254
CGGTCCA4405.627322E-5100.49053295
CATCGGA2800.002821059797.98352275-279
AGCGGGT4655.64507E-795.08781295
TTGCACG2952.6774456E-494.66369285-289
GACGCGG3000.004725453488.59883255-259
TTGACGG3250.00856954885.92551285-289
GGGTTCG3202.5956979E-982.515465250-254
CGGTTCG1050.003468148279.66139180-184
TCCGGTT3402.7052138E-678.175446255-259
Produced by FastQC (version 0.11.5)