FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2874714
Sequences flagged as poor quality0
Sequence length39-301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1809.142454E-4287.2614235-239
CGTAGGG3000.00370436179.99503280-284
TAAGCGG1609.949721E-4168.74533280-284
TGCGACG1050.0046768654166.60855250-254
GGTTACG1850.0067870975147.07544285-289
CGCCGAT2001.0525222E-5136.04478285-289
TATCGAC1904.3657034E-5135.44644230-234
TTACCGG2408.997784E-4112.06503275-279
TAACCGG2455.8260215E-5110.201035280-284
CGCGAGA1250.0051680943106.458595265-269
AACGTCG1200.0040759565102.25198110-114
GTACTCG2554.2740357E-699.54967205-209
CGCTTAA2604.0316995E-496.84576195-199
ATAACCG2655.145665E-496.84575225-229
GCGGTAC2850.003281788493.384735265-269
GATCGTT2700.002175173392.10038170-174
GTCGCCG3000.088.96897270-274
TAGGTCG1050.001877893383.85388260-264
ATGCGAG3250.00872415178.60661215-219
GCTATGC7058.265542E-572.47419215-219
Produced by FastQC (version 0.11.5)